prkcb  (Bioss)

Journal: Journal of Gastrointestinal Oncology

Article Title: Identifying drug candidates for pancreatic ductal adenocarcinoma based on integrative multiomics analysis

doi: 10.21037/jgo-23-985

Figure Lengend Snippet: Analysis of coexpression network and hub genes. (A) Cluster dendrogram of 5,696 coexpressed genes, which are significantly related to 4 hub genes. Nine modules were obtained. Enrichment analysis of the 4 modules, in which PHF12 belonged to the green module (B), FXYD3 to the yellow module (C), PRKCB to the brown module (D), and ZNF582 to the turquoise module (E). *, P<0.05; **, P<0.01; ***, P<0.001. KEGG, Kyoto Encyclopedia of Genes and Genomes.

Article Snippet: The proteins were treated with primary rabbit polyclonal antibodies ( PHF12 , SC-514864, 200 µg/mL, Santa Cruz; FXYD3 , AB205534, 1:1,000, Abcam; PRKCB , BS-0267R, 1:1,000, Bioss) and GAPDH (GB12002, 1:1,000, Servicebio, Wuhan, China), then with a horseradish peroxidase-conjugated goat anti-rabbit secondary antibody (GB23303, 1:3,000, Servicebio).

Techniques:

Journal: Journal of Gastrointestinal Oncology

Article Title: Identifying drug candidates for pancreatic ductal adenocarcinoma based on integrative multiomics analysis

doi: 10.21037/jgo-23-985

Figure Lengend Snippet: Measurement of PHF12, FXYD3 and PRKCB protein levels in our cohort. (A) The mean protein expression levels of PHF12, FXYD3 and PRKCB in PDAC were all significantly higher than those in adjacent nontumor tissue by immunohistochemistry. (B) Representative immunohistochemical staining images of PHF12, FXYD3 and PRKCB, among which PHF12 is primarily localized in the cell nucleus, and FXYD3 and PRKCB are both primarily expressed in the cytoplasm and cytomembrane. Original magnification: ×200. (C) Representative Western blotting images show that the proteins PHF12, FXYD3 and PRKCB were all overexpressed in PDAC tissue compared with normal adjacent tissue. (D) Western blotting analysis demonstrates that the mean grayscale values of PHF12, FXYD3 and PRKCB are all higher in fresh-frozen PDAC tissues than in matched adjacent normal tissues. MAD, mean areal density; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PDAC, pancreatic ductal adenocarcinoma.

Article Snippet: The proteins were treated with primary rabbit polyclonal antibodies ( PHF12 , SC-514864, 200 µg/mL, Santa Cruz; FXYD3 , AB205534, 1:1,000, Abcam; PRKCB , BS-0267R, 1:1,000, Bioss) and GAPDH (GB12002, 1:1,000, Servicebio, Wuhan, China), then with a horseradish peroxidase-conjugated goat anti-rabbit secondary antibody (GB23303, 1:3,000, Servicebio).

Techniques: Expressing, Immunohistochemistry, Immunohistochemical staining, Staining, Western Blot

Journal: Frontiers in Cell and Developmental Biology

Article Title: Single-Cell RNA Sequencing Profiles Identify Important Pathophysiologic Factors in the Progression of Diabetic Nephropathy

doi: 10.3389/fcell.2022.798316

Figure Lengend Snippet: Validation of the expression of the mTOR pathway markers in diabetic nephropathy. (A,B) Box plots of the expression of mTOR pathway markers EIF4B, RICTOR, and PRKCB in control and diabetic nephropathy samples in the (A) GSE111154 and (B) GSE142025 datasets. DN: diabetic nephropathy. (C) H&E staining of the morphology of kidney tissues in the control group and the diabetic nephropathy rat model group. Scale bar, 100 μm; magnification, ×400. (D) The mRNA expression of EIF4B, RICTOR, and PRKCB was determined in kidney tissues from the control and diabetic nephropathy groups through RT-qPCR. (E,F) The expression of EIF4B, RICTOR, and PRKCB proteins was determined in kidney tissues from the control and diabetic nephropathy groups through Western blot. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: Antibodies included EIF4B (1/10000; ab134138; Abcam, United States), RICTOR (1/1000; ab219950; Abcam, United States), PRKCB (1/2000; ab195039; Abcam, United States), and GAPDH (1/10000; ab8245; Abcam, United States).

Techniques: Expressing, Staining, Quantitative RT-PCR, Western Blot

Journal: Journal of ethnopharmacology

Article Title: Mechanism by which Huoxue Tongluo Qiwei Decoction improves the erectile function of rats with diabetic erectile dysfunction.

doi: 10.1016/j.jep.2021.114674

Figure Lengend Snippet: Fig. 7a. Electrophoretogram of four proteins (DAG, PKCβ, NF-κB, ICAM-1) in rats from the K, M, L, D, G groups. Fig. 7b. The expression levels of four proteins (DAG, PKCβ, NF-κB, ICAM-1) in rats from the K, M, L, D, G groups determined using western blotting. Fig. 7c.The mRNA expression levels of four proteins (DAG, PKCβ, NF- κB, ICAM-1mRNA) in rats from the K, M, L, D, G groups determined using RT-qPCR. Data are expressed as the mean ± SEM. Multiple comparisons analysis were conducted using one-way ANOVA. Differences with P < 0.05 were considered statistcally significant.*P < 0.05, the M group versus the Z group; #p < 0.05, the L, D, G groups vs the M group.

Article Snippet: Meanwhile, We purchased anti-DAG antibody (Bs-14190R, Woburn, MA, USA), anti-PKC antibody (Bs-0267R, Woburn, MA, USA), anti–NF–κB antibody (Bs- X. Li et al. Journal of Ethnopharmacology 283 (2022) 114674 23217R, Woburn, MA, USA), anti-ICAM-1 antibody (Bs-4617R, Woburn, MA, USA), and HRP (Bs-0295G, Woburn, MA, USA) from Rabbit Bioss.

Techniques: Expressing, Western Blot, Quantitative RT-PCR